A short-term suspension culture system for bovine oviduct epithelial cells suitable for the study of embryo-maternal communication processes
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منابع مشابه
A bovine oviduct epithelial cell suspension culture system suitable for studying embryo-maternal interactions: morphological and functional characterization.
We established a short-term (24 h) culture system for bovine oviduct epithelial cells (BOECs), obtained on day 3.5 of the estrous cycle and evaluated the cells with respect to morphological criteria, marker gene expression, and hormone responsiveness. BOEC sheets were isolated mechanically from the ampulla with similar yields from oviducts ipsi- and contralateral to the ovulation site (57.9 +/-...
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Background The oviduct epithelium consists of ciliated and secretory cells which play an important role in key reproductive processes such as sperm capacitation, fertilization and early embryonic development. Bovine oviduct epithelial cells have been widely used in co-culture experiments to condition culture media and improve early embryonic development [1]. However, these cells dedifferentiate...
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